Blocking-permeabilization solution for IF and IFC

Reagent	Final concentration	Amount
BSA	2%	1 gr
Triton X-100 (10% solution)	0.1% (v/v)	0.5 mL
PBS	n/a	45.5 mL
Total	n/a	50 mL

Prepare freshly on the day of staining for optimal results.