REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-GM130 antibody (EP892Y) Rabbit (1:100)	Abcam	Cat#ab52649
Anti-GM130 mAb (Monoclonal) (1:100)	MBL	Cat#M179-3
Goat anti-Rabbit IgG (H+L) Cross-adsorbed Secondary antibody, Alexa Fluor 568 conjugate (1:400)	Thermo Fisher Scientific	Cat#A-11011
Goat anti-Mouse IgG (H+L) Cross-adsorbed Secondary antibody, Alexa Fluor 568 conjugate (1:400)	Thermo Fisher Scientific	Cat#A-11004
DAPI 4′,6-Diamidino-2-phenylindole dihydrochloride (1:250)	Sigma-Aldrich	Cat#D9542
Chemicals, peptides, and recombinant proteins
DMEM (Dulbecco’s Modified Eagle Medium)	Gibco	Cat#41965-039
Fetal Bovine Serum, certified, South America	Gibco	Cat#12657-029
PBS (Phosphate Buffered Saline)	Sigma-Aldrich	Cat#D8537
Trypsin EDTA Solution C (0.05% Trypsin)	Sartorius	Cat#03-053-1B
Penicillin-Streptomycin Solution	Sartorius	Cat#03-031-1B
L-Glutamine Solution	Sartorius	Cat#03-020-1B
Triton X-100	Sigma-Aldrich	Cat#X100
Albumin, Bovine Fraction V	MP Biomedicals	Cat#160069
Paraformaldehyde 32% solution	Electron Microscopy Sciences	Cat#15714
Nocodazole	Sigma-Aldrich	Cat#M1404
Thymidine	Sigma-Aldrich	Cat#T1895
Methanol Absolute AR	Bio-Lab	Cat#136805
Experimental models: Cell lines
HeLa cells	ATCC	Cat#CCL-2
Software and algorithms
ZEISS Observer.Z1 Confocal Spinning Disk microscope system with camera-Q IMAGING ROLERA	Carl Zeiss	N/A
Imaging software - ZEN	Carl Zeiss	Version 2.5 system https://www.zeiss.com/microscopy/int/products/microscope-software/zen.html#downloads
ImageStreamX mark II	Luminex	N/A
Acquisition software - INSPIRE	Amnis/part of Luminex.	Version 200.1.681.0 https://www.luminexcorp.com/imagestreamx-mk-ii/#software
Analysis software - IDEAS	Amnis/part of Luminex.	Version 6.3 https://denovosoftware.com/full-access/download-landing/
Other
60 × 15 mm Tissue Culture Dish	Falcon	Cat#353004
12 well cell culture plates. Flat Bottom with Lid	Costar	Cat#3513
Microscope Cover Slips diameter 18 mm #1	Menzel Glaser Gmbh	CB00180RA120MNZ
Elvanol Mounting Solution	Waterborne Inc	Cat#M102
Orbital-Mixer/Intelli-Mixer	Thomas Scientific	N/A