Figure 3.

DNA priming followed by cryopreserved radiation attenuated sporozoite (cryo-RAS) trap protects mice against Plasmodium yoelii sporozoite (Py spz) challenge 4–6 weeks post-vaccination. (A) Experimental design of prime-and-trap studies. (B) Results of protection studies after challenge with 1 × 10³ wild-type purified Py spz administered on Day 56 (left) or Day 70 (right). N = 15 mice across two experiments (N = 5 mice from one experiment for HK-spz and RAS trap only [no ggCSP prime] groups). Fractions above bars indicate number of animals protected out of total group size. Protection was assessed with thin blood smears on Days 3–14 post-challenge. Fisher Exact Test: 2 × 10⁴ fresh-RAS vs. all other groups. ns = P > 0.05. ** P < 0.005, **** P < 0.0001.