Figure 5.

DNA priming followed by Plasmodium yoelii (Py) fresh- or cryopreserved radiation attenuated sporozoite (cryo-RAS) trap +/−7DW8-5 induces durable protection in mice. (A) Experimental design of prime-and-trap studies. (B) Results of protection studies after challenge with 1 × 10³ wild-type purified Py sporozoite (spz) administered 4 months (Day 140) after trapping. N = 10 mice across two experiments. Fractions above bars indicate number of animals protected out of total group size. Protection was assessed with thin blood smears on Days 3–14 post-challenge. Fisher Exact Test: fresh-RAS vs. all other groups. ns = P > 0.05. **** P < 0.0001.