Extended Data Figure 3. Lrrc8c^−/− mice have normal T cell development.

(a-d) Cell numbers and proportion of thymocytes from WT and Lrrc8c^−/− mice. (a) Absolute cell numbers, (b) representative flow cytometry plots showing the proportion (c) and numbers (d) of thymocyte subsets DN1, DN2, DN3, DN4, DP, SP4⁺ and SP8⁺ cells. (e-j) Cell numbers and proportion of splenocytes from WT and Lrrc8c^−/− mice. (e) Absolute cell numbers, (f) representative flow cytometry plots showing the gating strategy to identify the different subsets of T cells in the spleen from WT and Lrrc8c^−/− mice. Proportion and absolute cell numbers of (g) CD3⁺ T cells, (h) CD4⁺ and CD8⁺ T cells, and (i,j) naïve CD44^loCD62L^hi, T central memory (T_CM) CD44^hiCD62L^hi, and T effector memory (T_EM) CD44^hiCD62L^lo T cell subsets in both CD4⁺ and CD8⁺ T cells. (k-m) Cell number and proportion of T cells in the lymph nodes (LNs) from WT and Lrrc8c^−/− mice. (k) Absolute cell numbers, and proportion and absolute cell numbers of (l) CD3⁺ T cells, and (m) CD4⁺ and CD8⁺ T cells. Data represent mean ± s.e.m. of 9 mice per genotype (a-j) and 7 mice per genotype (k-m), pooled from at least 4 independent experiments.