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. Author manuscript; available in PMC: 2022 Apr 8.
Published in final edited form as: Curr Protoc. 2021 Feb;1(2):e26. doi: 10.1002/cpz1.26

Table 1.

NIB inhibitor recipe

Inhibitor Type Initial Conc. Dil. Factor Final Conc. per 50mL per 10mL per 1mL
DTT Protease 1M 1000x 1mM 50μL 10μL 1μL
AEBSF Protease 200mM 400x 0.5mM 125μL 25μL 2.5μL
Microcystin-LR Phosphatase 2.5μM 500x 5nM 100μL 20μL 2μL
in 100% EtOH
Sodium Butyrate HDAC 5M 500x 10mM 100μL 20μL 2μL
Additive Type Initial Conc. Dil. Factor Final Conc. Per 50mL per 10mL per 1mL
NP-40 Alternative Detergent 10% 33.3x 0.30% 1.5mL 300μL 30μL