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Offline HPLC Parameters
| Sample Volume | 80 μl |
| Temperature | 4.0 [°C] |
| Collection Tube Max Volume | 0.20 [ml] |
| Column Oven Temperature | 37.0 [°C] |
| Buffer A | 5% ACN 0.2% TFA |
| Buffer B | 95% ACN 0.188% TFA |
| UV wavelength | 214 [nm] |
| Equilibration | Duration = 10.000 [min] |
| Flow Rate | 0.250 [ml/min] |
| Column | 218TP52 |
| Time: | %B: |
| 0 | 25.0 [%] |
| 60 | 60.0 [%] |
| 75 | 100.0 [%] |
| 80 | 100.0 [%] |
