Table 5.
Primer sequences of microbiota
| Gene | Primer(5′–3′) | Length | Annealing temperature (℃) | Accession number |
|---|---|---|---|---|
| Bacterium | F:CCTACGGGAGGCAGCAG | 181 bp | 60 | * |
| R:TTACCGCGGCTGCTGG | ||||
| Rf | F:TATCTTAGTGGCGGACGGGT | 157 bp | 60 | MT356193.1 |
| R:TCTAATCAGACGCGAGCCCA | ||||
| Fs | F:GATGAGCTTGCGTCCGATT | 110 bp | 60 | EU606019.1 |
| R:ATTCCCTACTGCTGCCTCC | ||||
| Ra | F:GGGCTTAACCCCTGAACTGC | 114 bp | 60 | X85098.1 |
| R:TCGCCACTGATGTTCCTCCT | ||||
| Ram | F:GGGGACAACACCTGGAAACG | 124 bp | 60 | Y15992.1 |
| R:CTTGGTAGGCCGTTACCCCA | ||||
| Bf | F:CCTGACTAAGAAGCACCGGC | 107 bp | 60 | U41167.1 |
| R:GTAAAACCGCCTACGCTCCC | ||||
| MB | F:TCTGTACGGGTTGTGAGAGCA | 106 bp | 60 | KP752401.1 |
| R:CGCGATTTCTCACATTGCGG |