Table 3.
Saccharification rates (%) of cellulose and hemicellulose in glucose, xylose and arabinose and production of inhibitors after acid hydrolysis procedures, as well for enzymatic hydrolysis (means ± SD). Remaining cellulose and hemicellulose in samples after hydrolysis processes and conversion rate (%) of cellulose and hemicellulose in glucose and xylose.
Acid hydrolysis | |||||
---|---|---|---|---|---|
Sugars/Compounds | Acid concentration (%) | Saccharification rates (%) |
|||
Sulphuric acid |
Acetic acid |
||||
15 min | 60 min | 15 min | 60 min | ||
Glucose | 0.5 | 1.6 ± 0.03 | 2.4 ± 0.04 | 0.1 ± 0.01 | 0.12 ± 0.08 |
2 | 4.8 ± 0.01 | 5.4 ± 0.02 | 0.1 ± 0.05 | 0.12 ± 0.05 | |
3.5 | 6.1 ± 0.03 | 6.6 ± 0.09 | 0.08 ± 0.03 | 0.1 ± 0.09 | |
Xylose | 0.5 | 22.1 ± 0.6 | 21.0 ± 0.06 | 0.22 ± 0.01 | 0.05 ± 0.01 |
2 | 31.2 ± 0.02 | 34.7 ± 0.03 | 0.05 ± 0.07 | 0.11 ± 0.05 | |
3.5 | 38.0 ± 0.09 | 40.1 ± 0.01 | 0.71 ± 0.03 | 0.16 ± 0.07 | |
Arabinose | 0.5 | 12.5 ± 0.05 | 32.2 ± 0.04 | 4.47 ± 0.01 | 5.4 ± 0.03 |
2 | 26.8 ± 0.01 | 52.7 ± 0.07 | 8.04 ± 0.04 | 14.3 ± 0.05 | |
3.5 | 27.7 ± 0.06 | 81.3 ± 0.01 | 8.04 ± 0.03 | 11.6 ± 0.08 | |
HMF | 0.5 | 0.083 ± 0.97 | 0.077 ± 1.46 | 0.086 ± 1.23 | 0.088 ± 0.29 |
2 | 0.084 ± 0.11 | 0.078 ± 1.94 | 0.087 ± 0.17 | 0.090 ± 0.55 | |
3.5 | 0.087 ± 1.77 | 0.097 ± 1.95 | 0.089 ± 0.56 | 0.094 ± 0.75 | |
Acetic acid | 0.5 | 1.1 ± 97.75 | 1.2 ± 138.50 | 0.3 ± 193.08 | 0.4 ± 177.69 |
2 | 1.3 ± 43.76 | 1.6 ± 4.61 | 1.5 ± 216.08 | 1.8 ± 176.04 | |
3.5 | 1.5 ± 7.46 | 2.5 ± 196.45 | 2.7 ± 166.78 | 2.9 ± 153.96 |
Enzymatic hydrolysis | ||
Procedure | Remaining concentration of Cellulose (g/L) | Remaining concentration of Hemicellulose (g/L) |
GSWE-red/ First | 6.06 ± 0.06 | 8.08 ± 0.06 |
GSWE-red/ Second | 2.15 ± 0.39 | 0.004 ± 0.15 |
Process | Glucose (%) | Xylose (%) |
First saccharification | 25.97 | 82.73 |
Second saccharification | 12.9 | 3.27 |
Total (some of two processes) | 38.87 | 86.00 |