Table 2.

Box-Behnken factorial experimental design for melanin pigment production by Hortaea werneckii AS1

Runs	Experimental parameters			Response (g/L)
	CaCl2 (g/L)	Trace element (mL/L)	Culture volume (mL/500mL)
1	0[0.75]	0[0.5]	0[150]	0.58
2	-1[0.375]	1[0.75]	0[150]	0.59
3	0[0.75]	-1[0.25]	-1[75]	0.44
4	1[1.125]	0[0.5]	-1[75]	0.51
5	0[0.75]	1[0.75]	-1[75]	0.41
6	1[1.125]	-1[0.25]	0[150]	1.01
7	-1[0.375]	-1[0.25]	0[150]	0.44
8	0[0.75]	1[0.75]	1[225]	0.8
9	1[1.125]	0[0.5]	1[225]	0.9
10	0[0.75]	0[0.5]	0[150]	0.69
11	0[0.75]	-1[0.25]	1[225]	0.77
12	-1[0.375]	0[0.5]	-1[75]	0.28
13	-1[0.375]	0[0.5]	1[225]	1
14	1[1.125]	1[0.75]	0[150]	0.98
15	0[0.75]	0[0.5]	0[150]	0.65