Fig. 2.

Sodium butyrate improved glucose homeostasis profile. (a) Blood glucose level during intraperitoneal glucose tolerance test (IPGTT) done by two way ANOVA along with Bonferroni post-hoc analysis (N = 6) (b) Area under the curve during ipgtt done by student’s t-test. (c) Blood insulin level during insulin tolerance test (N = 10) (d) Area under the curve during ITT. (e) FITC dextran permeability assay showed better intestinal integrity in NaB treated mice as compared to HFD mice. (f) Immuno-blots of epididymal WAT of HFD and NaB treated mice using antibodies specific for tight junction protein .i.e., occludin and ZO-1, β-Actin served as a loading control. (g & h) Quantification of protein level normalized by β-actin. #P < 0.05, ##P < 0.01, ###P < 0.001, *P < 0.05, **P < 0.01, * **P < 0.001 comparison between HFD and NaB) indicated significance and ns indicated non-significant.