Fig. 3.
Anti-inflammatory gene expression profile triggered by Sodium Butyrate. (a & b) Immune cells of eWAT were profiled using flow cytometry for pro-inflammatory M1(CD11c + ) macrophages. (c & d) Anti-inflammatory M2(CD206 + ) macrophages. (e & f) Polarization of macrophages (g) Decreased mRNA expression of pro-inflammatory gene expression profile after the treatment of NaB. (h) Anti-inflammatory gene expression profile showing increase in the mRNA expression of IL-4 and IL-10 as compared to HFD. Student’s t-test used to analyze the data. #P < 0.05, ##P < 0.01, ###P < 0.001 (comparison between control and HFD) *P < 0.05, **P < 0.01, * **P < 0.001 (comparison between HFD and NaB) indicated significance and ns indicated non-significant.