Fig. 2.

Bt rice transgenic inserts in chromosomes 2 and 3, drawn to scale. In this event, two copies of the transgenic insert were introduced, at two different places. As a result, the internal portion of the inserts are the same except for a small length difference because of short truncations at the ends, while the junction regions are different. Each copy consists of two transgenic expression cassettes, arranged in a head-to-head orientation: one for the expression of the insect resistance gene CS-Cry1B, directed by the P-ubiZM1 core promoter along with the L-ubiZM1 (leader) and I-ubiZM1 (the first intron) and T-nos terminator, and one for the expression of the hygromycin resistance gene CS-hpt, directed by the P-e35S promoter (the P-35S promoter preceded by a duplicated L-35S enhancer) and the T-35S terminator. Transgenic elements are described in Supplementary File 3.