FIGURE 4.

Single-cell sequencing of the neuronal cells marked by AAV2/9 virus in sciatic nerves of adult rats. (A) Single cell acquisition model of virus-labeled neuronal cells. (B) Single-cell sequencing of virus-marked cells. Single-cell sequencing of neuronal cells was conducted after 2 weeks of hSYN-promoter AAV2/9 virus infection. The hot map represents the transcriptome similarities between 78 GFP⁺ Stmn2⁺ cells, 13 GFP⁺ Stmn2^– cells, and 10 DRG cells. GFP⁺ cells were dissected from nerves injected with hSYN-promoter GFP AAV2/9 virus. DRG neurons were dissected from adult rat DRGs in vivo. A total of 84 cells from cultured sciatic nerves, seven cells from virus-injected sciatic nerves in vivo, and 10 cells from DRG neurons. (C) t-SNE map represents the subcluster analysis of the 78 GFP⁺ cells that expressed high levels of Stmn2. The four colors represent four different clusters. The pie diagrams represented the cell cycle analysis of those cells. A total of 74 cells from cultured sciatic nerves and four cells from virus-injected sciatic nerves in vivo. See also Supplementary Figure 1.