Fig. 8.

External validation of SNAP25 in molecular experiments. a Western blot analysis of SNAP25 between PCa and normal clinical samples. β-actin was used as an internal control. b Quantification of SNAP25 protein levels. (c) Immunofluorescence analysis of SNAP25 (green) in normal prostate cells and PCa cells. Scale bar, 50 μm. d The relative mean fluorescence in each group was calculated. Data were presented as the means ± SD, n = 3. *p < 0.05, *p < 0.01, and ns, p > 0.05, no significant difference