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. 2021 Dec 7;4:100061. doi: 10.1016/j.fochms.2021.100061

Table 2.

Different properties presented in mate-pair and paired-end strategies.

Different properties WGS-MP WGS-PE
Recommended sequencing depth About 5× genome size Not less than 10× genome size
Flanking sequence information Many and valuable Little
Insertion sites and flanking sequence validation Easy Very easy
Number of false positives Few Extremely high
True positives in total results High Intermediate
Evaluation of copy number Easy Easy
Plasmid backbone residue analysis Easy Easy