Fig. 4.

An Immunofluorescence observation of CRT (green fluorescence) exposure on the 4T1 cells surface after incubation with PBS, MPSNs and MPSNs@R837 with or without laser irradiation (808 nm, 0.6 W/cm²) (scale bar = 25 μm). B Mean fluorescence intensities of 4T1 determined by flow cytometry. C HMGB1 and D ATP levels of 4T1 cells after 24 h. All data are mean ± SD (n = 3), statistical significances were calculated via Student’s t test, *p < 0.05, **p < 0.01