Table 4. Sequences of Primers Used for qRT-PCR^a.

target gene	forward (5′–3′)	reverse (5′–3′)
claudin-3	CCGCTCGAGGCATCTTTTGGGTACCTTTGCG	GAAGATCTCCAGATGTTCTGCGACGTGATG
claudin-7	GGATTGGTCATCAGATTGTCACA′	TGGCAGGTCCAAACTCGTACT
ZO-2	CTGGTGGCAATGATGTCG	CCCGCACTAATCCTCTGAAA
occludin	TGGCTATGGAGGCGGCTATGG	AAGGAAGCGATGAAGCAGAAGGC
JAM-2	ATGCTGCTGCTGCTACACTACTT	TGACTTCTTGACGGTGGTCTTTT
MUC-3	CGTGGTCAACTGCGAGAATGG	CCTCTCGCATCTCTATCTCGGC
caspase-1	CTTGGAGACATCCTGTCAGGG	AGTCACAAGACCAGGCATATTCT
NLRP3	CCATCAATGCTGCTTCGACA	GAGCTGCCTAAGGGAGGTAG
IL-1β	CAACCAACAAGTGATATTCTCCATG	GATCCACACTCTCCAGCTGCA
TLR4	GCTCTCAGCCATCCACAAAG	GAGTCGGGAAGAGGAAGAGG
TNF-α	CTCATGCACCACCATCAAGG	ACCTGACCACTCTCCCTTTG
β-actin	GTGAAGGTGACAGCAGTCGGTT	GAGAAGTGGGGTGGCTTTTAGGA

^aZO-2, tight junction protein 2; JAM-2, junctional adhesion molecule 2; MUC-3, mucins 3; NLRP3, NOD-like receptor protein 3; TLR4, toll-like receptor 4; TNF-α, tumor necrosis factor α.