Skip to main content
. Author manuscript; available in PMC: 2023 Mar 1.
Published in final edited form as: Neuropharmacology. 2021 Nov 29;205:108907. doi: 10.1016/j.neuropharm.2021.108907

Table 1.

Two-Way ANOVA

Two-way ANOVA
Figure Sex Assay Line # Factors F P value
1 Time × Dose (20, 175) = 8.035 <0.0001
1A Male vF 2 Time (3, 111) = 153.0 <0.0001
3 Dose (4, 35) = 107.4 <0.0001
4 Time × Dose (20, 170) = 5.054 <0.0001
1B Female vF 5 Time (4, 137) = 14.56 <0.0001
6 Dose (4, 34) = 30.99 <0.0001
Male vs Female 7 Dose × Sex (4, 68) = 39.70 <0.0001
1D vF 8 Dose (4, 68) = 129.6 <0.0001
9 Sex (1, 68) = 198.3 <0.0001
10 Time × Dose (12, 87) = 4.785 <0.0001
1E Male HP 11 Time (2, 71) = 154.9 <0.0001
12 Dose (4, 29) = 7.088 0.0004
13 Time × Dose (12, 102) = 4.579 <0.0001
1F Female HP 14 Time (2, 79) = 33.11 <0.0001
15 Dose (4, 34) = 18.84 <0.0001
Male vs Female 16 Dose × Sex (4, 62) = 11.76 <0.0001
1H HP 17 Dose (4, 62) = 18.31 <0.0001
18 Sex (1, 62) = 74.83 <0.0001
19 Time × Drug (5, 90) = 3.137 P=0.0118
2A Male vF 20 Time (3, 62) = 1.276 0.29
21 Drug (1, 18) = 8.300 0.0099
22 Time × Drug (5, 90) = 3.491 0.0063
2B Female vF 23 Time (2, 44) = 4.177 0.0158
24 Drug (1, 18) = 35.83 <0.0001
Male vs Female 25 Drug × Sex (1, 18) = 6.431 0.0207
2C vF 26 Drug (1, 18) = 53.06 <0.0001
27 Sex (1, 18) = 1.410 0.2505
28 Time × Dose (6, 78) = 1.584 0.163
3A Male vF 29 Time (2, 38) = 2.513 0.073
30 Dose (1, 13) = 4.781 0.0477
31 Time × Dose (6, 102) = 7.490 <0.0001
3B Female vF 32 Time (2, 43) = 10.87 <0.0001
33 Dose (1, 17) = 28.00 <0.0001
Male vs Female 34 Dose × Sex (1, 30) = 7.854 0.0088
3C vF 35 Dose (1, 30) = 33.18 <0.0001
36 Sex (1, 30) = 5.184 0.0301
37 Time × Dose (6, 72) = 0.4550 0.8392
3D Male Act 38 Time (3, 36) = 0.9631 0.4214
39 Dose (1, 12) = 2.480 0.1413
3E Female Act 40 Time × Dose (6, 96) = 2.553 0.0245
41 Time (3, 54) = 4.441 0.0054
42 Dose (1, 16) = 47.70 <0.0001
Male vs Female 43 Dose × Sex (1, 28) = 7.723 0.0096
3F Act 44 Dose (1, 28) = 35.91 <0.0001
45 Sex (1, 28) = 11.23 0.0023
46 Time × Drug (10, 70) = 0.4995 0.8847
4A Male vF 47 Time (2, 31) = 1.181 0.3246
48 Drug (2, 14) = 1.331 0.2956
49 Time × Drug (10, 95) = 1.212 0.2936
4B Female vF 50 Time (3, 63) = 4.286 0.0063
51 Drug (2, 19) = 4.712 0.0218
Male vs Female 52 Sex × Drug (2, 33) = 2.495 0.0979
4C vF 53 Drug (2, 33) = 1.286 0.2898
54 Sex (1, 33) = 4.205 0.0483
55 Time × Drug (10, 60) = 0.6296 0.7827
4D Male Act 56 Time (2, 31) = 2.162 0.1186
57 Drug (2, 12) = 1.014 0.3919
58 Time × Drug (10, 90) = 1.247 0.273
4E Female Act 59 Time (3, 61) = 2.143 0.0966
60 Drug (2, 18) = 2.662 0.0971
Male vs Female 61 Sex × Drug (2, 30) = 2.418 0.1062
4F Act 62 Drug (2, 30) = 3.854 0.0324
63 Sex (1, 30) = 3.383 0.0758
64 Strain × Drug (3, 28) = 3.545 P=0.0271
6A Male HP 65 Drug (3, 28) = 1.502 P=0.2358
66 Strain (1, 28) = 38.03 P<0.0001
67 Strain × Drug (3, 28) = 9.185 P=0.0002
6B Male CP 68 Drug (3, 28) = 13.28 P<0.0001
69 Strain (1, 28) = 45.09 P<0.0001
70 Strain × Drug (3, 32) = 0.5121 P=0.6768
6C Male Glucose 71 Drug (3, 32) = 1.046 P=0.3856
72 Strain (1, 32) = 1.417 P=0.2426
Male vs Female 73 Sex × Drug (1, 40) = 2.695 0.1085
6F HP 74 Drug (1, 40) = 59.53 <0.0001
75 Sex (1, 40) = 1.096 0.3015
Male vs Female 76 Sex × Drug (1, 40) = 2.946 0.0938
6I PP 77 Drug (1, 40) = 40.99 <0.0001
78 Sex (1, 40) = 0.3639 0.5497
Male vs Female 79 Sex × Drug (1, 36) = 4.876 0.0337
8C HP 80 Drug (1, 36) = 56.38 <0.0001
81 Sex (1, 36) = 1.812 0.1867
82 Time × Dose (15, 95) = 0.9696 0.4928
9A Male HP 83 Time (3, 71) = 5.808 0.0006
84 Dose (3, 19) = 0.7648 0.5278
85 Time × Dose (15, 90) = 1.232 0.2633
9B Female HP 86 Time (3, 68) = 5.721 0.0006
87 Dose (3, 18) = 1.805 0.1824
9C Male vs Female HP 88 Dose × Sex (3, 38) = 1.329 0.2793
89 Dose (3, 38) = 0.5167 0.6733
90 Sex (1, 38) = 0.1684 0.6839
Food intake 91 Time × Dose (12, 32) = 3.299 0.0034
9D Male 92 Time (1, 12) = 2.826 0.1042
93 Dose (3, 8) = 0.5317 0.6732
Food intake 94 Time × Dose (12, 40) = 7.158 <0.0001
9E Female 95 Time (1, 12) = 5.565 0.0313
96 Dose (3, 10) = 1.177 0.3667
Male vs Female Food intake 97 Dose × Sex (3, 24) = 0.9784 0.4194
9F 98 Dose (3, 24) = 6.669 0.002
99 Sex (1, 24) = 13.65 0.0011
100 Time × Dose (18, 114) = 28.95 <0.0001
9G Male Weight 101 Time (1, 32) = 13.10 0.0001
102 Dose (3, 19) = 2.476 0.0926
103 Time × Dose (18, 108) = 2.660 0.001
9H Female Weight 104 Time (1, 23) = 2.000 0.1685
105 Dose (3, 18) = 0.7926 0.5138
Male vs Female 106 Dose × Sex (3, 40) = 4.008 0.0138
9I Weight 107 Dose (3, 40) = 26.60 <0.0001
108 Sex (1, 40) = 2.142 0.1512
109 Time × Dose (18, 114) = 5.692 <0.0001
9J Male Glucose 110 Time (4, 80) = 13.36 <0.0001
111 Dose (3, 19) = 6.770 0.0027
112 Time × Dose (18, 108) = 2.754 0.0006
9K Female Glucose 113 Time (3, 68) = 4.003 0.0065
114 Dose (3, 18) = 1.110 0.3708
Male vs Female 115 Dose × Sex (3, 40) = 0.7509 0.5283
9L Glucose 116 Dose (3, 40) = 8.885 0.0001
117 Sex (1, 40) = 0.6510 0.4245

vF: von Frey; HP: Hotplate; Act: Acetone testing; PP: Paw pressure