Table 2.

Three-Way ANOVA

Figure	Sex	Assay	Line #	Factors	F	P value
			1	Time	(5, 168) = 150.6	<0.0001
			2	Sex	(1, 168) = 192.5	<0.0001
5A	Male vs Female	Body mass	3 4	Strain Time × Sex	(1, 168) = 480.0 (5, 168) = 3.609	<0.0001 0.004
	Male vs Female	Body mass	5	Time × Strain	(5, 168) = 1.257	0.2848
			6	Sex × Strain	(1, 168) = 63.98	<0.0001
			7	Time × Sex × Strain	(5, 168) = 1.413	0.2221

			8	Time	(5, 134) = 18.54	<0.0001
			9	Sex	(1, 134) = 0.8059	0.371
	Male vs Female	Blood Glucose	10	Strain	(1, 134) = 692.4	<0.0001
5B			11	Time × Sex	(5, 134) = 0.8510	0.516
			12	Time × Strain	(5, 134) = 27.32	<0.0001
			13	Sex × Strain	(1, 134) = 0.6879	0.4083
			14	Time × Sex × Strain	(5, 134) = 0.9537	0.4487

			15	Time	(5, 140) = 12.81	<0.0001
			16	Sex	(1, 28) = 0.1101	0.7425
5C	Male vs Female	HP	17 18	Strain Time × Sex	(1, 28) = 214.0 (5, 140) = 0.6481	<0.0001 0.6634
	Male vs Female		19	Time × Strain	(5, 140) = 3.431	0.0059
			20	Sex × Strain	(1, 28) = 6.489	0.0166
			21	Time × Sex × Strain	(5, 140) = 1.671	0.1454

			22	Time	(5, 168) = 2.939	0.0143
			23	Sex	(1, 168) = 0.3942	0.5309
5D	Male vs Female	PP	24 25	Strain Time × Sex	(1, 168) = 41.49 (5, 168) = 0.2074	<0.0001 0.959
	Male vs Female		26	Time × Strain	(5, 168) = 4.309	0.001
			27	Sex × Strain	(1, 168) = 0.3558	0.5517
			28	Time × Sex × Strain	(5, 168) = 0.7538	0.5844

			29	Time	(2, 90) = 2.437	0.0932
			30	Strain	(1, 45) = 79.43	<0.0001
			31	Drug	(1, 45) = 17.50	0.0001
6D	Male	HP	32	Time × Strain	(2, 90) = 0.8989	0.4106
			33	Time × Drug	(2, 90) = 6.504	0.0023
			34	Strain × Drug	(1, 45) = 1.735	0.1944
			35	Time × Strain × Drug	(2, 90) = 0.3000	0.7415

			36	Time	(2, 82) = 2.594	0.0809
			37	Strain	(1, 41) = 108.2	<0.0001
			38	Drug	(1, 41) = 15.24	0.0003
6E	Female	HP	39	Time × Strain	(2, 82) = 3.787	0.0267
			40	Time × Drug	(2, 82) = 7.700	0.0009
			41	Strain × Drug	(1, 41) = 4.647	0.037
			42	Time × Strain × Drug	(2, 82) = 0.4271	0.6538

6G	Male	PP	43	Time	(2, 86) = 5.997	0.0036
			44	Strain	(1, 43) = 268.9	<0.0001
			45	Drug	(1, 43) = 12.69	0.0009
			46	Time × Strain	(2, 86) = 2.513	0.087
			47	Time × Drug	(2, 86) = 2.950	0.0576
			48	Strain × Drug	(1, 43) = 2.641	0.1114
			49	Time × Strain × Drug	(2, 86) = 0.6298	0.5351

			50	Time	(2, 82) = 0.08181	0.9215
			51	Strain	(1, 41) = 82.62	<0.0001
			52	Drug	(1, 41) = 8.638	0.0054
6H	Female	PP	53	Time × Strain	(2, 82) = 3.253	0.0437
			54	Time × Drug	(2, 82) = 1.064	0.3496
			55	Strain × Drug	(1, 41) = 2.831	0.1001
			56	Time × Strain × Drug	(2, 82) = 7.442	0.0011

			57	Time	(2, 52) = 24.79	<0.0001
			58	Strain	(1, 26) = 206.3	<0.0001
7A	Male vs Female	Weight	59 60	Sex Time × Strain	(1, 26) = 10.81 (2, 52) = 3.369	0.0029 0.0421
	Male vs Female		61	Time × Sex	(2, 52) = 1.008	0.3719
			62	Strain × Sex	(1, 26) = 0.05295	0.8198
			63	Time × Strain × Sex	(2, 52) = 1.790	0.1771

			64	Time	(1, 50) = 233.8	<0.0001
			65	Strain	(1, 27) = 393.6	<0.0001
7B	Male vs Female	HbAlc	66 67	Sex Time × Strain	(1, 27) = 0.1071 (2, 54) = 168.3	0.746 <0.0001
	Male vs Female		68	Time × Sex	(2, 54) = 1.468	0.2394
			69	Strain × Sex	(1, 27) = 0.02360	0.879
			70	Time × Strain × Sex	(2, 54) = 0.006281	0.9937

			71	Time	(1, 50) = 60.23	<0.0001
			72	Strain	(1, 26) = 20.03	0.0001
7C	Male vs Female	HP	73 74	Sex Time × Strain	(1, 26) = 0.007916 (2, 52) = 5.446	0.9298 0.0071
	Male vs Female		75	Time × Sex	(2, 52) = 0.2999	0.7422
			76	Strain × Sex	(1, 26) = 2.385	0.1346
			77	Time × Strain × Sex	(2, 52) = 0.08583	0.9179

			78	Time	(2, 82) = 1.276	0.2877
			79	Strain	(1, 32) = 565.8	<0.0001
			80	Drug	(1, 32) = 17.42	0.0002
8A	Male	HP	81	Time × Strain	(3, 96) = 5.855	0.001
			82	Time × Drug	(3, 96) = 2.172	0.0963
			83	Strain × Drug	(1, 32) = 23.10	<0.0001
			84	Time × Strain × Drug	(3, 96) = 0.1530	0.9275

			85	Time	(2, 77) = 16.24	<0.0001
			86	Strain	(1, 32) = 174.4	<0.0001
8B	Female	HP	87	Drug	(1, 32) = 8.973	0.0053
			88	Time × Strain	(3, 96) = 9.445	<0.0001
			89	Time × Drug	(3, 96) = 7.931	<0.0001
			90	Strain × Drug	(1, 32) = 9.866	0.0036
			91	Time × Strain × Dru	(3, 96) = 8.165	<0.0001

HP: Hotplate; PP: Paw pressure