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. 2022 Mar 25;13:839524. doi: 10.3389/fmicb.2022.839524

FIGURE 1.

FIGURE 1

(A) Circos plot. The Circos plot represents the syntenic relationship between genomes of different AGs of Rhizoctonia solani Kühn. Each line represents the region of genomic similarity predicted with Synima. Only the regions with coverage > 40,000 bases were enumerated and shown. (B) The plot highlights the number of high-similarity syntenic regions (coverage > 40,000 bp) shared between each pair of genomes, including T. calospora. The red connection represents corresponding isolates sharing a comparatively large number of syntenic relationships relative to other pairs of isolates. Here, self-hits were removed or not shown. (C) ITS2 phylogeny. ITS2 sequences of the tester strain were obtained from the NCBI database and were clustered with ITS2 sequences from assembled R. solani genomes (highlighted with blue color and *), along with ITS2 sequences from previously published R. solani genome assemblies (marked with **). The phylogenetic tree was constructed using megax software with 10,000 bootstrapping steps (see section “Materials and Methods”), after which the resulting tree and corresponding alignment were visualized together using Phylogeny.IO.