Table 2. Kinetic Parameters of the Peroxidase Activity of hCyt c Variants at Different pH Values with Guaiacol as a Substrate.

	kcat (s–1)		Km (μM)
pH	I81N	WT	I81N	WT
5.0	0.39 ± 0.01	0.13 ± 0.01	36.9 ± 3	50.2 ± 9
6.0	0.25 ± 0.006	0.14 ± 0.03	21.2 ± 2	20.0 ± 1
7.0	0.16 ± 0.003	0.11 ± 0.002	30.5 ± 1	27.5 ± 1
8.0	0.066 ± 0.003	0.047 ± 0.002	28.1 ± 3	12.9 ± 3