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. Author manuscript; available in PMC: 2022 Jul 4.
Published in final edited form as: Sci Signal. 2022 Jan 4;15(715):eabh2290. doi: 10.1126/scisignal.abh2290

Fig. 1. DNA damage-induced AKT-Ser473 phosphorylation is dependent on mTORC2.

Fig. 1.

(A) MCF7 cells were irradiated (IR) with 10 Gy or treated with 10 μM etoposide (Eto) for indicated time points before harvesting for immunoblot (IB) analysis. n = 3 independent experiments. (B) Representative immunofluorescent images of AKT-pSer473 and γ-H2AX in MEFs irradiated with 10 Gy and recovered for 60 min. n = 2 independent experiments. Scale bar, 10 μm. (C) Sin1+/+ and Sin1−/− MEFs were irradiated with 10 Gy and then harvested in the indicated time points for IB analysis. n = 2 independent experiments. (D) Rictor+/+ and Rictor−/− MEFs were irradiated with 10 Gy and then harvested in the indicated time points for IB analysis. n = 2 independent experiments.