Figure 6.

Increase of gemcitabine sensitivity. (A) Effect on growth of SUIT-2 and Hs766t cells of the combination of gemcitabine at its IC₂₅, with the compounds IS1 and IS4 at their IC₅₀. The observed values were lower than the theoretical values. (B) Modulation of hENT1 mRNA levels. Expression was determined with quantitative-PCR by normalisation with the GAPDH housekeeping gene, as described in the methods. Since we previously demonstrated that hENT1 protein levels correlated with hENT mRNA expression, we did not include hENT1 protein expression^[71,72]. Columns, mean values obtained from triplicate experiments; bars, SEM; *P < 0.05.