Table 1.
BCNU-Resistance Genes
| Gene | Percent of Expression in Drug Naïve 9L-S | ||||
|---|---|---|---|---|---|
| 9L-R1 | 9L-R2 | 9L-R3 | mean ± SD | P-value | |
| CRABP1 | 0.97 | 0.97 | 2.25 | 1.40 ± 0.74 | 9.7 × 10-12 |
| NGF receptor | 3.99 | 3.99 | 3.56 | 2.43 ± 1.51 | 1 × 10-8 |
| NGF | 8.26 | 7.59 | 8.55 | 8.13 ± 0.49 | 2 × 10-9 |
| Brain creatine kinase | 9.07 | 8.67 | 9.65 | 8.87 ± 0.28 | 2.7 × 10-10 |
| VG Na channel, type 1 | 27.7 | 13.9 | 17.5 | 19.70 ± 7.16 | 1.5 × 10-6 |
| Na/Ca exchanger 5 | 35.7 | 44.9 | 39.7 | 40.1 ± 4.61 | 2.9 × 10-6 |
| FGF 9 | 423 | 818 | 275 | 505 ± 280 | 3.1 × 10-14 |
| TGF-β2 | 508 | 849 | 772 | 710 ± 179 | 4.5 × 10-11 |
| MGMT | 850 | 642 | 652 | 715 ± 117 | 1.1 × 10-8 |
| Galectin 3 | 3055 | 311 | 3342 | 2236 ± 1673 | 5.1 × 10-10 |
Microarray analysis was used to identify genes regulated in chemoresistant lines (9L-R1, 9L-R2, and 9L-R3) compared to the parent drug naïve line of 9L cells. NGF: nerve growth factor; VG Na channel; voltage-gated sodium channel; Na/Ca exchanger; sodium/calcium exchanger; FGF: fibroblast growth factor; TGF: transforming growth factor; MGMT: O-6-methylguanine-DNA methyltransferase