TABLE 2.

Identified significantly different metabolites from Enterotoxigenic Escherichia coli (ETEC) and ETEC + Sodium humate (HNa) groups under positive and negative ion model^a.

Adduct	Metabolites	VIP	FC	P-value	m/z	rt (s)
(M + H − H2O)+	1-Stearoyl-rac-glycerol	1.18	0.51	0.004	341.30	191.95
(M + CH3COO + 2H)+	2-Ethoxyethanol	1.18	0.78	0.008	151.09	65.48
(M + H)+	Trp-Arg	1.01	0.57	0.03	361.19	339.80
(M + H)+	Erucamide	1.69	1.44	0.03	338.34	34.44
(M + H − H2O)+	Val-Asp	2.63	0.25	0.03	215.10	342.13
(M + NH4)+	alpha-Linolenic acid	2.95	0.84	0.0	296.25	36.71
(M + H)+	Deoxycytidine	3.19	2.29	0.042	228.0	205.20
(M + H)+	Sphinganine	1.51	0.58	0.044	302.30	109.24
(M + CH3COO)−	Chenodeoxycholate	3.14	0.60	0.02	451.30	154.48
(M − H)−	Propionic acid	5.76	0.47	0.03	73.02	383.06
(M − H)−	Succinate	8.86	0.47	0.03	117.01	383.06
(M − H)−	Gentisic acid	1.68	1.72	0.03	153.01	70.10
(M − H)−	3-Phenylpropanoic acid	6.68	1.94	0.03	149.06	98.09
(M − H)−	17-glucosiduronate	1.25	0.43	0.03	447.20	127.10
(M − H)−	Isobutyric acid	11.46	1.81	0.04	87.04	122.35

Different metabolites identified by positive and negative ion mode (multi-dimensional statistical analysis of VIP > 1 and univariate statistical analysis of P-value < 0.05), the ETEC + HNa group versus the ETEC group.

^aAdduct, adduct ion information of the compound; VIP, variable importance in the projection; FC, Fold change; m/z, mass-to-charge ratio; Rt(s), retention time.