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. 2022 Jan 4;5(1):80–89. doi: 10.20517/cdr.2021.112

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© The Author(s) 2022.

© The Author(s) 2022. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, sharing, adaptation, distribution and reproduction in any medium or format, for any purpose, even commercially, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Schema for developing resistance to VAC therapy in mice using the single mouse study design. Mice received a single cycle of VAC (vincristine, actinomycin D, cyclophosphamide). Tumor response was determined, and tumors were transplanted in recipient mice when they achieved 400% of their volume on the first day of treatment.