Figure S3.

The neonatal liver is enriched with Ly49E⁺ ILC1s that are rarely replenished after birth. (A) Representative plots showing the gating strategy and characteristics of neonatal liver group 1 ILCs (0–3 d old). (B) Percentages of Ly49E⁺ ILC1s, Ly49E⁻ ILC1s, and cNK cells among liver group 1 ILCs from WT mice at different ages. Data for each time point are representative of three independent experiments with n = 3–6 mice. (C) Flow cytometric plots showing Ly49E expression among CD45⁺ leukocytes from neonatal liver (0–3 d old). Data are representative of at least three independent experiments. (D and E) Lethally irradiated recipient CD45.1⁺ mice received donor CD45.2⁺ BM cells (6–8 wk old) or CD45.2⁺ fetal liver cells (E13.5–15.5). 1 mo later, the livers of recipient mice were harvested for flow cytometric analysis of Ly49E expression on ILC1s (D). Percentages of BM- or fetal liver–derived Ly49E⁺ cells among donor-derived ILC1s (E). Data are representative of two independent experiments with n = 3 or 4 mice. (F) Absolute cell numbers of Ly49E⁻ ILC1s in the liver of WT and Klra5^DTR mice at 1 or 4 wk after intrahepatic (i.h.) treatment with DT once during the neonatal period (0–3 d old). Data were pooled from two or three independent experiments with n = 5–16 mice. Bar graphs show the mean ± SEM; unpaired Student’s t test was used in E, and one-way ANOVA followed by Dunnett’s multiple comparison test in F; ****, P < 0.0001.