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. Author manuscript; available in PMC: 2022 Oct 9.
Published in final edited form as: Anal Chim Acta. 2021 Jul 26;1181:338873. doi: 10.1016/j.aca.2021.338873

Figure 4. MS1 spectra of SAM and SAH from mouse liver extract and human plasma, with internal standards SAM-2H3 and SAH-2H4.

Figure 4.

Samples were prepared as described in the Experimental. a) Mouse liver extract spectrum comprising 69 FT spectra from over approx. 4 min acquisition under full scan MS1 100–1000 m/z at 1M resolution setting and divided by the number of summed scans to provide an average. The averaged peak intensity or area is normalized to that of the internal deuterated standard to determine the concentration. The region displayed is 384 – 403 m/z and the insets show 0.02 m/z windows for greater detail. Errors stated are accurate mass errors from theoretical m/z values. b) Human plasma spectrum of a pancreatic cancer patient. The spectrum comprises 82 spectra averaged over approx. 9 min acquisition under full scan MS1 150–1000 m/z at 1 M resolution setting. The averaged intensity is normalized to that of the internal deuterated standard to determine the concentration. The region displayed is 384 – 403 m/z and the insets show 0.02 m/z windows for greater detail. Errors stated are accurate mass deviation from theoretical m/z values.