TABLE 4.
Peptidoglycan precursors and vancomycin MICs for E. faecalis JH2-2 and derivatives under different growth conditions
| E. faecalis strain | Medium supplementa | Peptidoglycan precursors (%)b
|
Vancomycin MIC (μg/ml) | ||
|---|---|---|---|---|---|
| UDP-MurNAc-pentapeptide[Ala] | UDP-MurNAc-pentapeptide[Ser] | UDP-MurNAc-tetrapeptide | |||
| JH2-2 | None | 100 | 0 | 0 | 2 |
| JH2-2/pCA10 (vanC-1 vanXYC vanT) | None | 3 | 23 | 74 | 8 |
| JH2-2/pCA10 (vanC-1 vanXYC vanT) | d-Serine | 6 | 38 | 56 | 8 |
| JH2-2/pCA10 (vanC-1 vanXYC vanT) | l-Serine | 6 | 40 | 54 | 8 |
a
The concentrations of d- and l-serine were 50 mM.
b
Determined by HPLC from the integrated peak areas.