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Relative expression of mRNAs of the tight junction-related factors in the gill of on-growing grass carp fed diets containing various vitamin A levels for 10 weeks, which were then infected by F. columnare. The values shown are the means (n = 6); error bars indicate SD. β-actin was used as an internal control gene. Different letters indicate the significant difference (P < 0.05). ZO-1, zonula occludens-1; MLCK, myosin light chain kinase.
