Table 4. Test sensitivity and specificitya for in silico identification of antimicrobial resistance genes in Escherichia coli isolates from wildlife, swine manure pits, and environmental sources in southern Ontario, 2011–2013 (n = 200).
| Antimicrobial class | Test Sensitivity (95%CI) | Test Specificity (95%CI) |
|---|---|---|
| Aminoglycoside | 95.5% (89−99%) | 81.8% (73−88%) |
| Beta-lactam | 90.2% (82−96%) | 98.3% (94−99%) |
| Macrolide | 100% (16−100%) | 97.0% (93−99%) |
| Sulfonamide | 97.0% (90−97%) | 97.0% (92−99%) |
| Phenicol | 95.6% (78−99%) | 99.4% (97−99%) |
| Tetracycline | 98.6% (95−99%) | 96.2% (87−99%) |
| Overall b | 95.9% (93−98%) | 95.5% (94−97%) |
a Phenotypic antimicrobial resistance test results were considered the gold standard. Detection of 15 antimicrobials performed using the CMV3AGNF panel from National Antimicrobial Resistance Monitoring System (Sensititre, Thermo Scientific). In silico acquired resistance genes detected using Abricate and the Resfinder database.
b Raw counts for all isolates and antimicrobials were pooled together.