Figure 1.

LSH is a TET interacting partner.

(A) Co-immunoprecipitation between LSH and TET CD proteins. Flag-tagged TET1, TET2 and TET3 CDs were overexpressed in HEK293T cells and immunoprecipitated with an anti-FLAG antibody. Left panel: inputs. Right panel: Immunoprecipitates.(B) LSH and TET proteins interact in vitro. Top panel: Schematic representation of the LSH protein and truncated forms fused to GST and tested for TET interaction. Bottom left panel: GST pull down experiment between LSH and truncated LSH forms with CD of TET1, TET2 and TET3 produced by in vitro translation. Bottom right panel: Similar experiment with full-length TET1, TET2 and TET3.(C) Co-immunoprecipitation between LSH and TET proteins in ESCs. Cell lysates were subjected to immunoprecipitation with an anti-TET1 (left panel) or anti-TET2 (right panel) antibody and subjected to western blot using anti-LSH. Inputs and IgG controls are indicated. Figures were cropped and re-assembled from the same blot to remove irrelevant lanes for the current study (uncropped western blots are available in Supplemental Figure 1b).