| Strain, strain background (Japanese quail) |
Coturnix coturnix japonica
|
Moshav Mata |
NCBI taxon: 93,934 |
|
| Antibody |
anti-GFP (Rabbit polyclonal) |
Invitrogen, Thermo-Fisher Scientific |
Cat#A6455; RRID:AB_221570
|
IF(1:1000) |
| Antibody |
anti-GFP (Mouse monoclonal) |
Abcam |
Cat#Ab38689; RRID:AB_732715
|
IF(1:100)Not available anymore |
| Antibody |
anti-RFP (Rabbit polyclonal) |
Acris |
Cat# AP09229PU-N; RRID:AB_2035909
|
IF(1:1000) |
| Antibody |
anti-pSmad1/5/8 (guinea pig polyclonal) |
Ed Laufer |
N/A |
IF(1:300) |
| Antibody |
anti-pSmad1/5/9 (Rabbit monoclonal) |
Cell Signaling Technology |
Cat#CST13820; RRID:AB_2493181
|
IF(1:500) |
| Antibody |
anti-H3-pS10 (Mouse monoclonal) |
Abcam |
Cat#Ab14955; RRID:AB_443110
|
IF(1:400) |
| Antibody |
anti-Laminin (Rabbit polyclonal) |
Sigma, Israel |
Cat#L9393; RRID:AB_477163
|
IF(1:100) |
| Antibody |
anti-Sox9 (Rabbit polyclonal) |
Millipore |
Cat#AB5535; RRID:AB_2239761
|
IF(1:150) |
| Antibody |
anti-SNAI2 (Rabbit monoclonal) |
Cell Signaling Technology |
Cat#CST9585; RRID:AB_2239535
|
IF(1:500) |
| Antibody |
anti-CD57(HNK1) (Mouse monoclonal) |
BD Biosciences |
Cat#559048; RRID:AB_397184
|
IF(1:500) |
| Antibody |
anti-BarHL1 (Rabbit polyclonal) |
Sigma, Israel |
Cat# HPA004809; RRID:AB_1078266
|
IF(1:300) |
| Antibody |
anti-Pax7(Mouse monoclonal) |
DHSB |
Cat# pax7; RRID:AB_528428
|
IF(1:10) |
| Antibody |
anti-Hb9 (mouse monoclonal) |
DHSB |
Cat# 81.5C10; RRID:AB_2145209
|
IF(1:200) |
| Recombinant DNA reagent |
pCAGG(plasmid) |
Krispin et al., 2010b
|
|
|
| Recombinant DNA reagent |
pCAGGS-EGFP(plasmid) |
Krispin et al., 2010b
|
|
|
| Recombinant DNA reagent |
pCAG-mGFP(plasmid) |
Addgene |
RRID:Addgene_14757
|
|
| Recombinant DNA reagent |
pCAGGS-RFP(plasmid) |
Ofek et al., 2021
|
|
|
| Recombinant DNA reagent |
pCAGGS-RARα403(plasmid) |
This paper |
|
Subcloned as described in Methods section |
| Recombinant DNA reagent |
pCAGGS-Cyp26A1(plasmid) |
This paper |
|
Subcloned as described in Methods section |
| Recombinant DNA reagent |
pCAB-cSmad6(plasmid) |
Nitzan et al., 2016
|
|
|
| Recombinant DNA reagent |
pCAG-VP16-RARα-IRES-eGFP(plasmid) |
Novitch et al., 2003
|
|
From S. Sockanathan |
| Recombinant DNA reagent |
pGL3-RARE-SV40-AP(plasmid) |
Gupta and Sen, 2015
|
|
From J. Sen |
| Recombinant DNA reagent |
pGL3-RARE-SV40-RFP(plasmid) |
This paper |
|
Subcloned as described in Methods section |
| Recombinant DNA reagent |
pGL3-RARE-SV40-d2EGFP (plasmid) |
This paper |
|
Subcloned as described in Methods section |
| Recombinant DNA reagent |
12XTOPFLASH‐d2EGFP (plasmid) |
Rios et al., 2010
|
|
|
| Recombinant DNA reagent |
cRaldh2 (plasmid) |
Diez del Corral et al., 2003
|
|
From K. StoreyTemplate for probe synthesis |
| Recombinant DNA reagent |
cBAMBI (plasmid) |
Casanova et al., 2012
|
EST 603482731F1 |
From A. Sanz-EzquerroTemplate for probe synthesis |
| Recombinant DNA reagent |
cCyp26A1 (plasmid) |
Wilson et al., 2007
|
|
From R. WingateTemplate for probe synthesis |
| Recombinant DNA reagent |
cHairy1 (plasmid) |
Jouve et al., 2000; Nitzan et al., 2016
|
|
From D. HenriqueTemplate for probe synthesis |
| Recombinant DNA reagent |
cfoxd3 (plasmid) |
Dottori et al., 2001
|
EST 603374321F1 |
From M. CheungTemplate for probe synthesis |
| Recombinant DNA reagent |
cRARα (plasmid) |
Diez del Corral et al., 2003
|
|
From K. StoreyTemplate for probe synthesis |
| Recombinant DNA reagent |
cRARβ (plasmid) |
Diez del Corral et al., 2003
|
|
From K. StoreyTemplate for probe synthesis |
| Recombinant DNA reagent |
cRARγ(plasmid) |
A.Graham |
|
Template for probe synthesis |
| Recombinant DNA reagent |
cRXRα (plasmid) |
A.Graham |
|
Template for probe synthesis |
| Recombinant DNA reagent |
cRXRγ (plasmid) |
A.Graham |
|
Template for probe synthesis |
| Sequence-based reagent |
qCyp1B1_F |
This paper |
PCR primers |
GTGTTGTGACTGCTGGGATG |
| Sequence-based reagent |
qCyp1B1_R |
This paper |
PCR primers |
AGATTGACCAGTGAGCCAGG |
| Sequence-based reagent |
qsox9_F |
This paper |
PCR primers |
TCGAAGGAAACTGGCTGACC |
| Sequence-based reagent |
qsox9_R |
This paper |
PCR primers |
ATCAATGTGGGGAGGTTGGC |
| Sequence-based reagent |
qRspo1_F |
This paper |
PCR primers |
AAACCACCGGTCTCTGTGTC |
| Sequence-based reagent |
qRspo1_R |
This paper |
PCR primers |
AGCAGGAGGGAAGGAAGAAG |
| Sequence-based reagent |
qdraxin_F |
This paper |
PCR primers |
TGTGCTGGATGTGGTTGTTT |
| Sequence-based reagent |
qdraxin_R |
This paper |
PCR primers |
TGGTTTGCAGAGATGCTCAC |
| Sequence-based reagent |
qGrem1_F |
This paper |
PCR primers |
AGGCTGCTTTTGGAGAACAA |
| Sequence-based reagent |
qGrem1_R |
This paper |
PCR primers |
GAATGGGTTTTGGTTGATG |
| Sequence-based reagent |
qCRABP1_F |
This paper |
PCR primers |
ACCTGGAAGATGAGGAGCAG |
| Sequence-based reagent |
qCRABP1_R |
This paper |
PCR primers |
CACACGGTCACATACAACACC |
| Sequence-based reagent |
qNorrin(NDP)_F |
This paper |
PCR primers |
GCACTGTCCTAAAGCAGCCT |
| Sequence-based reagent |
qNorrin(NDP)_R |
This paper |
PCR primers |
TTCAGGCCCCGGGAGATATT |
| Sequence-based reagent |
qsnai2_F |
This paper |
PCR primers |
TGAGATACGGGGAAAGACGC |
| Sequence-based reagent |
qsnai2_R |
This paper |
PCR primers |
AGGCACTTGGAGGGGTAATG |
| Commercial assay or kit |
KAPA2G Fast ReadyMix PCR |
Sigma, Israel |
Cat#KK5101 |
|
| Chemical compound, drug |
NBT |
Roche |
Cat#11383213001 |
|
| Chemical compound, drug |
BCIP |
Sigma, Israel |
Cat#B8503 |
|
| Chemical compound, drug |
Fast Red |
Sigma, Israel |
Cat#F4648 |
|
| Software, algorithm |
FIJI |
Schindelin et al., 2009
|
https://imagej.net/software/fiji/
|
|
| Software, algorithm |
R and R-Studio |
https://www.R-project.org; http://www.rstudio.com
|
RRID:SCR_000432
|
R Version 4.0.3 |
| Software, algorithm |
Adobe Photoshop and Indesign CS6 |
Adobe |
https://www.adobe.com/
|
|
| Software, algorithm |
Graphpad Prism |
Graphpad https://www.graphpad.com
|
RRID:SCR_002798
|
Version 7.0 |
| Other |
BTX square wave electroporator |
BTX, San Diego, CA, USA |
Cat#45–0662 |
|
| Other |
DP73 cooled CCD digital camera |
Olympus |
https://www.olympus-global.com/
|
|
| Other |
BX51 microscope |
Olympus |
https://www.olympus-global.com/
|
|
| Other |
Nikon Eclipse 90i microscope |
Nikon |
https://www.nikon.com/
|
|
| Other |
D-Eclipse c1 confocal system |
Nikon |
https://www.nikon.com/
|
|
| Other |
DIG RNA mix |
Roche |
Cat#11277073910 |
|
| Other |
anti-digoxigenin-AP |
Roche |
Cat#11093274910 |
|
| Other |
Hoechst 33,258 stain |
Sigma, Israel |
Cat#14,530 |
(125 ng/ml) |
