Table 1.

The infectivity change of mutant SARS-CoV-2 virus evaluated by MuCov pipeline and pseudotyped virus experiments.

Mutation	BFEa of wild-type	BFEa of mutation	P-valueb	Infectivity predicted by MutCov	Infectivity verified by Experimentsd
V341I	−1063.82	−968.03	2.43E-05	Decreasec	Decrease
N343Q	−1063.82	−954.17	1.53E-08	Decreasec	Decrease
D364Y	−1063.82	−1139.9	2.28 E−02	Increase	Decrease
385-387del	−1063.82	−580.49	9.05E-14	Decreasec	Decrease
D405V	−1063.82	−1404.58	1.21E-13	Increase	Decrease
Q414P	−1063.82	−952.87	2.68E-06	decreasec	decrease
I434K	−1063.82	−1140.21	2.98E-04	Increase	Decrease
S438F	−1063.82	−1056.02	1.65E-03	Decreasec	Decrease
D467V	−1063.82	−1066.04	0.80	Increase	Decrease
P491R	−1063.82	−1285.05	2.61E-11	Increase	Decrease
V503F	−1063.82	−905.23	1.91E-09	Decreasec	Decrease
R509K	−1063.82	−960.91	0.00348688	Decreasec	Decrease
V510L	−1063.82	−821.38	6.63E-14	Decreasec	Decrease
P521S	−1063.82	−945.07	1.61E-09	Decreasec	Decrease

^aThe binding free energy (kJ/mol) of RBD region of S protein (wild-type or mutant) binding to ACE2.

^bThe p value of Wilcoxon rank-sum statistic for the binding energy change between wild-type and mutation.

^cThe antigenicity predicted by MutCov show a consistent change with pseudotyped virus experiments.

^dThe infectivity verified by pseudotyped virus experiments.