Figure 1.
Atm-null mice have abnormal respiratory mechanics in naïve mice. WT and Atm-null mice were infected with 105 plaque-forming units (PFUs) of HKx31. Naïve and HKx31 infected WT (black bars) and Atm-null (gray bars) mice were ventilated on a Flexivent small animal ventilator. Lung function was analyzed on naïve mice and at 14 and 56 dpi. A: total respiratory system resistance (Rrs), Newtonian resistance (RN; B), and compliance (Crs; C) were graphed. D: pressure-volume (PV) curve from naïve WT and Atm-null mice. Values represent means ± standard error with individual values shown as dots. E: lungs were harvested from naïve mice and at 7, 14, and 56 dpi. Representative images of lung sections from WT (top) and Atm-null (bottom) mice stained with hematoxylin and eosin. Inset boxes are enlarged in the lower images. Arrows indicate regions of epithelial thinning. Arrowheads indicate regions of epithelial thickening. Scale bar = 100 µm and inset scale bar = 200 µm. Representative images of n ≥ 3 mice/time point/group are shown. P values were determined by Student’s t test. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001; n ≥ 3/time point/group. dpi, days postinfection; HKx31, Hong Kong/X31; WT, wildtype.