TABLE 1.
Primer name | Sequence |
---|---|
miR‐182‐5p |
Forward: GGCGGTTTGGCAATGGTAGA Reverse: AGTGCAGGGTCCGAGGTA Stem: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAGTGTG |
miR‐9‐5p |
Forward: CGGCGGTCTTTGGTTATCTAGC Reverse: AGTGCAGGGTCCGAGGTA Stem: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTCATAC |
TLN1 |
Forward: TACAGTCAGCCAAGGAGGTG Reverse: AGGAATGCTGGAGAACTCAGG |
UTR TLN1 |
Forward: CCCTCGAGGGAGAGCTTCGAGATGAGCACT Reverse: ATTTGCGGCCGCGGCTCAGACACTTACAGGC |
Over miR‐182‐5p |
Forward: CCCTCGAGGGGGACCTTGTCGCAGTTGC Reverse: CGGAATTCCGACCTGCCCTCTGCCACTAC |
pEGFPN1vector |
Forward: TGTCGTAACAACTCCGCCC Reverse: TGAACAGCTCCTCGCCCTT |
psiCHECK‐2 vector |
Forward: GAGGACGCTCCAGATGAAATG Reverse: CTCACACAAAAAACCAACACACAG |
Mutant UTR |
Forward: GCCACTACCAAAGCCTTCTCCAGTCCCGCAGTACATC Reverse: GATGTACTGCGGGACTGGAGAAGGCTTTGGTAGTGGC |