Fig. 4.

Immune-inflammatory proteomic profile is observed in Ly6ChiCD11bhi cells but not Ly6ChiCD11blo cells. a A scree plot for the PCA is presented. b Projection plot for PC1 and PC2. c Projection plot for PC1 and PC3. d Gene concept network of proteins in enriched categories in the bottom 5% of PC2 loading scores (characteristic for inflamed Ly6C^hiCD11b^hi cells). e Proteins in enriched categories in the top 5% of PC3 loading scores (characteristic for control Ly6C^hiCD11b^lo cells). The Benjamini–Hochberg FDR was set to 0.05 for all GO analyses (see detailed results in Fig. S4). f Heatmap of selected key proteins residing in either the PC1 or PC2 bottom 5% or PC3 top 5% of loading scores. g Pearson coefficient and R² heatmap of PC loadings vs. the cMoP/Ly6C signature (log₂FC) reported by Hettinger et al., 2013¹⁸. h Scatterplot of PC1 and PC2 loadings vs. the cMoP/Ly6C signature (log₂FC) (340 proteins detected in both datasets). Quadrant counts indicated (top right). i Cell surface expression of the markers CD177, MHCII and TGF-β in control and inflamed Ly6C^hiCD11b^hi and Ly6C^hiCD11b^lo cells. Positive controls are indicated by asterisks