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. 2022 Apr 8;8:179. doi: 10.1038/s41420-022-00926-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Three Three METTL3 siRNAs (si-METTL3#1, si-METTL3#2, and si-METTL3#3) were transfected into U2OS cells with relatively high METTL3 expression, with si-NC as negative control. pcDNA 3.1 METTL3 (pc-METTL3) was transfected into HOS cells with relatively low METTL3 expression, with pc-NC as negative control. After 48 h, A, B METTL3 expression in OS cells was detected using RT-qPCR and western blot. Two siRNAs with high transfection efficiency (si-METTL3#1 and si-METTL3#2) were selected for subsequent experimentation. B–D The proliferation of OS cells was measured using CCK-8 assay (B), colony formation assay (14 days) (C), and EdU staining (D). The cell experiment was repeated three times independently. Data are presented as mean ± standard deviation. Data in panels A, B/D–E were analyzed using one-way ANOVA, and data in panel C were analyzed using two-way ANOVA, followed by Tukey’s multiple comparisons test, *p < 0.05, **p < 0.01.