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. 2022 Apr 8;8:179. doi: 10.1038/s41420-022-00926-5

Fig. 5. HDAC5 reduced the acetylation of miR-142 promoter and thereby inhibited miR-142-5p expression.

Fig. 5

A H3/H4ac level in human OS cell lines (U2OS, HOS, SAOS2) and normal osteoblasts (hFOB1.1) was detected using western blot. B, C The enrichment of HDAC5 and H3K9/K14ac on miR-142 promoter in human OS cell lines (U2OS, HOS, SAOS2) and normal osteoblasts (hFOB1.1) was analyzed using ChIP-qPCR. D miR-142-5p expression in 50 pairs of OS tissues and adjacent tissues was determined using RT-qPCR. E miR-142-5p expression in human OS cell lines (U2OS, HOS, SAOS2) and normal osteoblasts (hFOB1.1) was determined using RT-qPCR. F The correlation between miR-142-5p and HDAC5 and METTL3 in OS tissues was analyzed using Pearson correlation analysis. G miR-142-5p expression in OS cells after intervention with METTL3 and HDAC5 expression was measured using RT-qPCR. N = 50. The cell experiment was repeated three times independently. Data in panels A–C/E/G are presented as mean ± standard deviation. Data in panel D were analyzed using paired t-test. Data in panels B/E/G were analyzed using one-way ANOVA, and data in panels A/C were analyzed using two-way ANOVA, followed by Tukey’s multiple comparisons test, *p < 0.05, **p < 0.01.