Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Apr 8;8:179. doi: 10.1038/s41420-022-00926-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — A The target genes of miR-142-5p were predicted through Starbase, miRWalk, Targetscan, and miRDB databases. B The binding site of miR-142-5p and ARMC8 in the Starbase database. C, D The binding of miR-142-5p and ARMC8 in OS cells was verified using dual-luciferase assay and RNA pull-down. E ARMC8 mRNA level in 50 pairs of OS tissues and adjacent tissues was determined using RT-qPCR. F ARMC8 mRNA level in human OS cell lines (U2OS, HOS, SAOS2) and normal osteoblasts (hFOB1.1) was determined using RT-qPCR. G The correlation between ARMC8, miR-142-5p, HDAC5, and METTL3 in OS tissues was analyzed using Pearson correlation analysis. H ARMC8 mRNA level in OS cells after intervention with METTL3 and HDAC5 expression was measured using RT-qPCR. N = 50. The cell experiment was repeated three times independently. Data in panels C-D/F-H are presented as mean ± standard deviation. Data in panel E were analyzed using paired t-test. Data in panels F/H were analyzed using one-way ANOVA, and data in panels C, D were analyzed using two-way ANOVA, followed by Tukey’s multiple comparisons test, *p < 0.05, **p < 0.01.