Fig. 8. METTL3 silencing suppressed OS proliferation in vivo via the HDAC5/miR-142-5p/ARMC8 axis.

The xenograft tumor model in nude mice was established with U2OS cells stably low-expressing METTL3. A Tumor volume recorded every week. B After the nude mice were euthanized on the 35th day, the tumor weight and representative images were presented. C The positive rate of Ki67 was detected using immunohistochemistry. D, E METTL3 level was determined using RT-qPCR and immunohistochemistry. F m⁶A level was quantitatively analyzed. G, H HDAC5 level was determined using RT-qPCR and immunohistochemistry. I, J miR-142-5p and ARMC8 level was determined using RT-qPCR. K ARMC8 level was detected using immunohistochemistry. N = 6. The cell experiment was repeated three times independently. Data in panels A–C/E, F/H are presented as mean ± standard deviation. Data in panels B–K were analyzed using t-test. Data in panel A were analyzed using two-way ANOVA, followed by Tukey’s multiple comparisons test, **p < 0.01.