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. 2022 Apr 8;13(4):321. doi: 10.1038/s41419-022-04782-0

Fig. 4. S1-OPA1 overexpression induced mitochondrial superoxide generation, mitochondrial bioenergetic deficits, and mitochondrial apoptosis after OGD/R.

Fig. 4

A Representative images of Mito-Sox staining in different overexpression groups under normal condition and OGD/R for 24 h. B Quantitative analysis of superoxide generation in neurons through red fluorescence intensity. C ATP content in neurons of each group was leveled by protein concentration. D Western blot analysis of caspase-9 and cleaved caspase-9 in neurons. E Ratio of cleaved caspase-9 to caspase-9 was quantitatively analyzed to represent the level of mitochondrial apoptosis. ***p < 0.001 vs. control group, ###p < 0.001, ##p < 0.01, #p < 0.05, $$$p < 0.001, $$p < 0.01, $p < 0.05; scale bar = 20 μm; n = 3.