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. 2022 Apr 8;13(4):322. doi: 10.1038/s41419-022-04732-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Verification of hsa_circRNA_0088036 knockdown by qRT-PCR analysis. B T24 and UMUC3 cells were transfected with hsa_circRNA_0088036-shRNA. Cell growth was measured with a cell proliferation assay. C, D T24 cells (C) and UMUC3 cells (D) were transfected with hsa_circRNA_0088036-shRNA. Cell migration was measured with the wound healing assay. E, F T24 cells (E) and UMUC3 cells (F) were transfected with hsa_circRNA_0088036-shRNA. 100 μL matrigel was added into upper chambers. Transwell invasion assays were performed. The invading cells were counted in 10 randomly chosen microscopic fields (100×) of each experiment and pooled. G SUSD1 expression in 72 paired human BCa and adjacent normal bladder tissues. H Correlation analysis of SUSD1 mRNA and hsa_circRNA_0088036 level was performed with the Pearson’s correlation coefficient. I The qRT-PCR analysis was performed to detect the expression of SUSD1 mRNA after overexpression of hsa_circRNA_0088036 in T24 cells and knockdown of hsa_circRNA_0088036 in UMUC3 cells. J Western blot analysis was performed to detect the expression of SUSD1 after overexpression of hsa_circRNA_0088036 in T24 cells and knockdown of hsa_circRNA_0088036 in UMUC3 cells.