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. 2022 Apr 8;13(4):322. doi: 10.1038/s41419-022-04732-w

Fig. 3. Hsa_circRNA_0088036 upregulates FOXQ1 expression through miR-140-3p in BCa cells.

Fig. 3

A The addition of miR-140-3p mimics partially rescued the growth of UMUC3 cells transfected with functional FOXQ1-cDNA, as determined by the cell proliferation assay. B The addition of a miR-140-3p inhibitor partially rescued the growth of UMUC3 cells transfected with FOXQ1-shRNA, as determined by the cell proliferation assay. C Transwell invasion assays were performed using four groups of T24 cells (pWPI+NC, oeFOXQ1, oemiR-140-3p, and oeFOXQ1+oemiR-140-3p). 100 μL matrigel was added into upper chambers. The invading cells were counted in 10 randomly chosen microscopic fields (100×) of each experiment and pooled. D Transwell invasion assays were performed using four groups of UMUC3 cells (pLKO+NC, shFOXQ1, shmiR-140-3p, and shFOXQ1+shmiR-140-3p). 100 μL matrigel was added into upper chambers. The invading cells were counted in 10 randomly chosen microscopic fields (100×) of each experiment and pooled. (E, F) Western blot analysis was performed to detect the expression of FOXQ1 after knockdown (E) and overexpression (F) of hsa_circRNA_0088036 in T24 and UMUC3 cells. G The qRT-PCR analysis was performed to detect the expression of FOXQ1 mRNA after knockdown of hsa_circRNA_0088036 in T24 and UMUC3 cells. H, I The qRT-PCR analysis was performed to detect the expression of miR-140-3p after knockdown of hsa_circRNA_0088036 in T24 cells (H) and overexpression of hsa_circRNA_0088036 in UMUC3 cells (I). J, K An argonaute2 immunoprecipitation assay was performed after knockdown of hsa_circRNA_0088036 in T24 (J) and UMUC3 (K) cells. L, M Co-transfection of wild type or mutant seed regions of FOXQ1 3’-UTR constructs with miR-140-3p in T24 (L) and UMUC3 cells (M). The luciferase assay is applied to detect luciferase activity.