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. 2022 Apr 8;12:5976. doi: 10.1038/s41598-022-10048-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Generation of a large set of stable haploid S. eubayanus strains. (a) Experimental procedure designed to generate the set of stable haploid S. eubayanus strains. A1. HO deletion, A2. Dissection of stable haploids and A3. Phenotyping of haploid strains. (b) Number of strains present in the wild collection of S. eubayanus, the sub-sets of null mutants and haploid strains generated (Created with BioRender.com). A single ‘MATa’ and ‘MATα’ spore was selected for phenotyping (c) Histogram of spore viability in 36 successfully transformed isolates (Δho).