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. 2022 Apr 8;8:176. doi: 10.1038/s41420-022-00987-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A The proliferation of MS751 cells with or without MAT2A depletion was examined by CCK8 assay under normal or glucose deprivation. Error bar mean ± s.d. ^*P < 0.05. ^###P < 0.001, ^#P < 0.05. B The proliferation of C33A cells with or without PDCD6 depletion was examined by CCK8 assay under normal or glucose deprivation. Error bar mean ± s.d. ^*P < 0.05. ^###P < 0.001, ^##P < 0.01, ^#P < 0.05. C The proliferation of MS751 cells with or without PDCD6 depletion was examined by CCK8 assay under normal or glucose deprivation. Error bar mean ± s.d. ^*P < 0.05. ^###P < 0.001, ^##P < 0.01, ^#P < 0.05. D Cell apoptosis of MS751 cells with or without MAT2A depletion was analyzed by Annexin V assay followed by flow cytometry (Left panel). Quantitative results were shown in Right panel. Error bar mean ± s.d. ^***P < 0.001, ^*P < 0.05. E, F Cell apoptosis of C33A cells (E) and MS751 cells (F) with or without PDCD6 depletion was analyzed by Annexin V assay followed by flow cytometry (Left panel). Quantitative results were shown in right panel. Error bar mean ± s.d. ^**P < 0.01. G–I Cell apoptosis was analyzed by immunoblotting with the indicated antibodies under normal or glucose deprivation in MS751 cells with or without MAT2A depletion (G), C33A cells with or without PDCD6 depletion (H) and MS751 cells with or without PDCD6 depletion (I).