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. 2022 Apr 8;8:176. doi: 10.1038/s41420-022-00987-6

Fig. 3. PDCD6 is methylation at lysine 90 and increases the protein stability.

Fig. 3

A MS751 cells with expressing 3×flag-MAT2A were cultured for 12 h under glucose deprivation. Immunoprecipitation was performed using Flag antibody, and the extracts were analyzed by mass spectrometry. The results of a mass spectrometric analysis revealed that 3 amino acids, R74, K77 and K90, of PDCD6 were methylated. B MS751 cells transfected with shMAT2A or 3×flag-PDCD6 were cultured for 12 h under normal or glucose deprivation. Cellular extracts were subjected to immunoprecipitation with an anti-methylated PDCD6 antibody. C MS751 cells were transfected a vector for control shRNA or PDCD6 shRNA and reconstituted with expression of WT rPDCD6, rPDCD6 R74K, rPDCD6 K77R or rPDCD6 K90R. Cell lysates were directly subjected to immunoblotting with the indicated antibodies. D MS751 cells were transfected with a vector for control shRNA or PDCD6 shRNA and reconstituted with expression of WT rPDCD6, rPDCD6 R74K, rPDCD6 K77R or rPDCD6 K90R. Cellular extracts were subjected to immunoprecipitation with an anti-arginine or lysine methylation antibody.