Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Apr 8;12:5975. doi: 10.1038/s41598-022-10066-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Automated axon identification. (A) Part of a cross-section of a rat tibial nerve in a late regeneration stage, 12 weeks post-surgery, 7 mm distal to the nerve coaptation. Myelinated axons are shown as black circles. Osmium tetroxide postfixed, epoxy embedded, 1 µm thickness. A myelin split artifact and prominent unmyelinated axons are present in this section. (B) Manual segmentation of the image in A by a blinded investigator. Myelin split artifacts and unmyelinated axons are correctly classified. Although an irregularly shaped, compressed nerve fiber is correctly identified as such, its myelin sheath is not fully segmented (dashed circle). (C) Automated segmentation of the image in A using AxonDeepSeg. The myelin split artifact (white arrowhead) as well as prominent unmyelinated axons (white arrows) have been misinterpreted as myelinated axons. Myelin sheaths are shown in red and axons are shown in blue. Scale bar represents 5 µm. (Own illustration created with AxonDeepSeg).