Fig. 3.

Overview angiogenesis and vasculogenesis patterning configurations and sample output. A Schematic diagram of an angiogenesis assay. 1 µL of acellular fibrin gel is patterned in the central lane (red), followed by 2.5 µL of lung fibroblasts in fibrin gel (green) in the right side lane, and 2.5 µL of HUVEC suspension deposited as a confluent monolayer is seeded in the left (blue) side lane. Flow from the LF to EC compartments is induced by adding media to the LF side reservoir. Adjusted standard deviations for Area, Total Length, and Branches are: 0.1118, 0.0626, and 0.2047, respectively (n = 28). B Sample 28 well imaging output of angiogenesis within a single injection molded chip, center lane (red) was selected as ROI. C Schematic diagram of a vasculogenesis assay. 1 µL of HUVECs suspended in fibrin gel is patterned in the central lane (red), followed by 2.5 µL of LFs in fibrin gel in both the right (green) and left (blue) side lanes. Adjusted standard deviations for Area, Total Length, and Junctions are: 0.0596, 0.0276, and 0.0594, respectively (n = 28). D Sample 28 well imaging output of vasculogenesis within a single injection molded chip, center lane (red) was selected as ROI. The measurement was performed after 7 days of cultivation